Single molecule analysis of bacterial polymerase chain reaction products in submicrometer fluidic channels
نویسندگان
چکیده
منابع مشابه
Microchip Electrophoresis and the Analysis of Polymerase Chain Reaction Products
and control provided by miniature electronic devices (7). However, DNA samples in biological fluids often are present at concentration levels that are too low for any direct test. Therefore, analysts normally use enzyme-induced amplification such as polymerase chain reaction (PCR) to increase the sample concentration before analysis (8,9). In this article, I will describe the use of microchip-b...
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Gene expression is a dynamic process involving messenger ribonucleic acid (mRNA) quantities that can vary from tens to millions. In this work, single molecule quantification [1, 2] of a sample’s genetic content is performed in a fluidic channel with submicrometer (500 nm by 250 nm) cross-sectional dimensions. Molecular beacon (MB) probes are utilized to increase robustness and selectivity in de...
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BACKGROUND Microbiological investigations of vitreous fluid (VF) and aqueous humour (AH) specimens have often failed to detect the infecting agent in infectious endophthalmitis, resulting in a clinical dilemma regarding therapy. In this study, the polymerase chain reaction (PCR) was evaluated in the diagnosis of bacterial and Propionibacterium acnes endophthalmitis. METHODS 58 intraocular spe...
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ژورنال
عنوان ژورنال: Biomicrofluidics
سال: 2007
ISSN: 1932-1058
DOI: 10.1063/1.2789565